how to get rid of non specific bands in PCR
Posted 29 June 2011 - 07:36 PM
Posted 29 June 2011 - 10:03 PM
Reducing Mg++, increasing annealing temp, increasing/ decreasing extension time and temperature are different suggestions that can be made depending on where your un specific bands are.
For bands lower than your band of interest, increase annealing temp, for those higher, decrease extension time.
NEW!!!! RotM interview with Dr. D. Allan Drummond about tRNA modification on CoffeeTableScience!!!!
Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist