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RNA-EMSA with Proteins which bind non-specifically to RNA


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#1 Jamsheer Muhammed

Jamsheer Muhammed

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Posted 29 June 2011 - 02:46 AM

hi everyone,
I'm working on plant proteomics and I'm going to do RNA-EMSA with my protein. in silico analysis shows that the mammalian homologues of my protein bind to RNA non-specifically. So, firstly I need to confirm it. I want to know that is there is any type of RNA which is routinely used for this purpose (like ΦX174 RF I and ΦX174 virion DNA used for DNA-EMSA)...I want both ss and dsRNA probe.....
thanks..
jamsheer

Edited by Jamsheer Muhammed, 29 June 2011 - 02:46 AM.





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