cell number for colony formation
Posted 28 June 2011 - 10:53 PM
I have a basic question. I am doing the colony formation assay using HEK293 or NIH3T3 cells. How many cells (HEK293 or NIH3T3) should I seed for this assay? I have tried 3000 HEK293 cells in a well of a 6-well plate, but that was too many...
Posted 29 June 2011 - 01:32 AM
Edited by bob1, 29 June 2011 - 01:47 AM.