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cell number for colony formation


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#1 lical208

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Posted 28 June 2011 - 10:53 PM

Hi everybody,

I have a basic question. I am doing the colony formation assay using HEK293 or NIH3T3 cells. How many cells (HEK293 or NIH3T3) should I seed for this assay? I have tried 3000 HEK293 cells in a well of a 6-well plate, but that was too many...
Thanks.

#2 bob1

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Posted 29 June 2011 - 01:32 AM

Well, less than 3000 obviously - I would try 500-1000. You may want to try conditioning the medium too.

Edited by bob1, 29 June 2011 - 01:47 AM.





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