Ligating Incompatible DNA fragments
Posted 28 June 2011 - 09:54 PM
I am trying to ligate my gene into a new vector. In order to cut my gene out of the current plasmid I have used SmaI and SacI restriction endonuclease.
The vector i want to insert my gene in is designed to be cut with BsmBI. So my question is, is there a way for me to cut my insert with SmaI and SecI and then ligate it with the vector which has been cut with BsmBI?
I know those enzymes are incompatible. If there is a way, could someone please tell me how?
Thank you in advance.
Posted 29 June 2011 - 01:27 AM
Posted 29 June 2011 - 01:54 AM
Edited by GNANA, 29 June 2011 - 01:55 AM.
I always had an alternate hypothesis....
Posted 29 June 2011 - 05:24 AM