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Total RNA estimation


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#1 Thapa

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Posted 28 June 2011 - 08:06 AM

In my new lab, RNA estimation is carrying out using 96 well plate. Simply add 99 ul of DEPC-H20 and 1 ul RNA sample. So, after reading at 260, the RNA is estimated as ug/ul=OD at 260 nm*40*dilution/1000

My questions are:
1. how much accuracy we get when we use 96 well plate (polysterine or else but not quartze cuvette)?

2. the pathlength 1 cm is required for 40, right? Im not sure 100 ul volume is standard for that??

Best,
Thapa
"Learning without thought is labor lost"

#2 pcrman

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Posted 08 July 2011 - 08:27 PM

What instrument do you use to measure the RNA concentration? Nanodrop or what?

#3 Thapa

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Posted 17 July 2011 - 11:02 AM

What instrument do you use to measure the RNA concentration? Nanodrop or what?


Simply a Platereader (SpectraMax, Molecular Devices) and in 96 well plate.
"Learning without thought is labor lost"




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