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Reference protein for membrane proteins in SDS-PAGE

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#1 Inmost sun

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Posted 28 June 2011 - 06:18 AM

often used reference proteins for cytosolic proteins are b-actin or a-tubulin; however, which reference protein to use for membrane fraction? receptor proteins are not suitable as they normally have a vivid turnover. what about membrane skeleton proteins (ankyrin, spectrin, ezrin protein family, other actin-binding proteins etc)? Or is also b-actin to use as it is linked to the plasma membrane?

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