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FPLC buffer exchange; 8 M urea to 8 M guanidine hydrochloride

Started by dustbunny000, Jun 27 2011 12:56 PM

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#1 dustbunny000

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Posted 27 June 2011 - 12:56 PM

I want to use gel filtration on FPLC to exchange an 8 M urea buffer into a 8 M guanidine hydrochloride buffer. Does anyone know if there's a way to tell if all the urea has been exchanged?

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