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fixing/storage of cells for later analysis with Pi/Ax - possible?


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#1 greenhorn

greenhorn

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Posted 27 June 2011 - 10:05 AM

Hello everyone,

So far I did apoptosis screening with PI/Ax FACS-analysis myself with cells fresh from culture stained with the Annexin V-FITC apoptosis detection kit from BenderMedSystems containing PI and Ax.
Now I just moved to an institution where FACS is done by one central facility, which is not open on week-ends and is closed for 2 weeks in august. So no FACS analysis during these times.

So here's the question:
Is there a way to store the cells between collection and FACS-analysis without impacting the result (stain, fix, store and wash/ fix and store and wash and stain later/....)? Any help would save me a lot of time!!

Thank you very much!!




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