I am about beginnig to chromotinimmunoprecipitation (ChIP) experiments
and it is indicated that to fixation I must use FORMALDEHYDE.
I want to your help espacially who is applying the ChIP or ChIP-on-ChIP experiments..
As you know, formaldehyde is toxic compond. I need to know that how I can use it
during my studies and what I must consider when I use it in the lab..
Thanks a lot...
Murat K. AVCI
Submit your paper to J Biol Methods today!
1 reply to this topic
Posted 16 July 2011 - 07:49 PM
Formaldehyde is simply one of the nastiest chemicals around. See the attachment for info on how to protect yourself. I have done a lot of work with ChIP DNA so I feel your pain. I recommend using this [url="[url]http://www.alkaliscientific.com/en/pcr-dna-clean-up-concentration-kits/695-chip-dna-clean-concentrator.html"]ChIP[/url] DNA Clean-up & concentration kit[/url] for maximum yields.