2 replies to this topic

[Wisoot]

I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

[protolder]

Wisoot, on 26 June 2011 - 06:14 PM, said:

I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

Hola, yes plasmid are inside, and only you have a bit of bacteria broken, defrost the pellet and dissolve in the resuspension buffer, after with the lisys buffer you have a part of the work made. Buena suerte

[Wisoot]

Thanks a lot Veteran.