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Mini prep of frozen bacteria cells


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#1 Wisoot

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Posted 26 June 2011 - 06:14 PM

I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

#2 protolder

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Posted 26 June 2011 - 09:59 PM

I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?

Thank you.

Hola, yes plasmid are inside, and only you have a bit of bacteria broken, defrost the pellet and dissolve in the resuspension buffer, after with the lisys buffer you have a part of the work made. Buena suerte

#3 Wisoot

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Posted 26 June 2011 - 10:29 PM

Thanks a lot Veteran.




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