Mini prep of frozen bacteria cells
Posted 26 June 2011 - 06:14 PM
Posted 26 June 2011 - 09:59 PM
Hola, yes plasmid are inside, and only you have a bit of bacteria broken, defrost the pellet and dissolve in the resuspension buffer, after with the lisys buffer you have a part of the work made. Buena suerte
I did not spin down bacteria pellets before keeping into -21 C freezer for 2 days. Can I still do mini prep?