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How to keep the shape of transfected Hela cell?


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#1 eagleleec

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Posted 14 October 2002 - 10:46 AM

Hi,
I have transfected Hela cell with Invitrogen Plus and Lipofect system, The eficiency is very high, almost 60%. But all the transfected Hela cell become round, I can't identify the cytoplasm and nuclear. Who can tell me how can I keep the shape of transfected ahela cell? Thanks a lot. [SIZE=7][COLOR=green]

#2 warp

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Posted 07 May 2003 - 07:13 AM

Hi,
It is known that liposomes are creating some problems when entering the cell membrane.. It may be the cause of your problem! Have you tried a non liposomal reagent ? I love jetpei (it's PEI but works great !).
Hope this helped !

WARP




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