I am using pTRKH3 plasmid http://bccm.belspo.b...s.php?NM=pTRKH3 .
I am trying to clon a cDNA (fused to GFP) in pTRKH3 but when I do the transformation (using DH5alpha) with Erythromycin (150ug/ml or 250ug/ml) the plate is full of bacteria (there are no colonies).
Could you tell me what's going on?
Many thanks in advance,
Erythromycin LB agar
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