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Erythromycin LB agar


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#1 valen

valen

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Posted 23 June 2011 - 05:09 AM

Hi,
I am using pTRKH3 plasmid http://bccm.belspo.b...s.php?NM=pTRKH3 .
I am trying to clon a cDNA (fused to GFP) in pTRKH3 but when I do the transformation (using DH5alpha) with Erythromycin (150ug/ml or 250ug/ml) the plate is full of bacteria (there are no colonies).

Could you tell me what's going on?

Many thanks in advance,
Valen




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