Dear all,
Who knows what is the best technique to determine mRNA concentration?
Thanks in advance
Peter
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welchtom
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Posted 22 October 2002 - 10:56 AM
Hey
Easiest way is to use a spectrophotometer. Take readings at both 260nm and 280 nm. Protocol is very common. Simple calculatoin to determine quantity of RNA, as well as purity!
Tom
Easiest way is to use a spectrophotometer. Take readings at both 260nm and 280 nm. Protocol is very common. Simple calculatoin to determine quantity of RNA, as well as purity!
Tom
