I am extracting RNA from plasma for microRNA microarray work. After the extraction and the total RNA was put for QC check on bioanalyzer. However, we can not see a good peck of 18S and 28S and the RIN is very low.
Is measuring 28S/18S in plasma a right way or not? If not, may I know the recommended alternatives for checking the RNA integrity?
Measuring 28S/18S in plasma is right or wrong?
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