Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Freezing Medium for MCF-10A cells


  • Please log in to reply
No replies to this topic

#1 Felix Broecker

Felix Broecker

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 22 June 2011 - 03:14 PM

Hi there,

I have a question about the freezing medium for MCF-10A. As some of you know, these cells require quite a fancy growth medium with EGF, Cholera toxin, hydrocortisone and insulin. ATCC recommends this complete growth medium, with the addition of 7.5% DMSO, to freeze the cells.

Now I have this really practical pre-mixed cell culture freezing medium called Recovery (Invitrogen), which is normal medium with DMSO, but certainly no EGF etc. Invitrogen says it works with a broad range of cells, and I used it with HeLa, 293T, HepG2 and it was fine.

So my question is whether any of you knows if I could use the convenient Recovery medium for my MCF-10A, without fancy supplements, to freeze the cells. Has anyone frozen MCF-10A in any freezing medium other than the complete medium w/ DMSO as recommended by ATCC, e.g., DMEM with serum and DMSO?

Thanks in advance for your answers!

Best
Felix




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.