3 replies to this topic

[labrat612]

What freezing density is best to use for NIH 3T3 cells?


Thanks.
Labrat612

[leelee]

I don't know about what is best, but we typically freeze a few million cells (btw 5 and 10) in a single aliquot of freezing medium (1ml). Seems to work just fine.

[rhombus]

labrat612, on 22 June 2011 - 10:46 AM, said:

What freezing density is best to use for NIH 3T3 cells?


Thanks.
Labrat612

I freeze down 1-3 million cells in 1.5 ml of freezing media. This allows me to resusitate cells into either a T25/T75 TC flask. NIH 3T3 cells are mouse fibroblasts which grow very well....so you might even get away with 500,000 cells/vial.

I rarely count my cells when freezing them down, estimating the cell number depending upon the surface area of the flask.

Hope this is useful.

Kindest regards.

Uncle Rhombus

[labrat612]

Thank you all for these suggestions! It is definitely much appreciated!!!