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Freezing density for NIH 3T3 cells


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3 replies to this topic

#1 labrat612

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Posted 22 June 2011 - 10:46 AM

What freezing density is best to use for NIH 3T3 cells?


Thanks.
Labrat612

#2 leelee

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Posted 22 June 2011 - 07:09 PM

I don't know about what is best, but we typically freeze a few million cells (btw 5 and 10) in a single aliquot of freezing medium (1ml). Seems to work just fine.

#3 rhombus

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Posted 24 June 2011 - 02:14 AM

What freezing density is best to use for NIH 3T3 cells?


Thanks.
Labrat612



I freeze down 1-3 million cells in 1.5 ml of freezing media. This allows me to resusitate cells into either a T25/T75 TC flask. NIH 3T3 cells are mouse fibroblasts which grow very well....so you might even get away with 500,000 cells/vial.

I rarely count my cells when freezing them down, estimating the cell number depending upon the surface area of the flask.

Hope this is useful.

Kindest regards.

Uncle Rhombus

#4 labrat612

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Posted 24 June 2011 - 05:06 AM

Thank you all for these suggestions! It is definitely much appreciated!!!




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