Freezing density for NIH 3T3 cells
Posted 22 June 2011 - 10:46 AM
Posted 22 June 2011 - 07:09 PM
Posted 24 June 2011 - 02:14 AM
I freeze down 1-3 million cells in 1.5 ml of freezing media. This allows me to resusitate cells into either a T25/T75 TC flask. NIH 3T3 cells are mouse fibroblasts which grow very well....so you might even get away with 500,000 cells/vial.
I rarely count my cells when freezing them down, estimating the cell number depending upon the surface area of the flask.
Hope this is useful.
Posted 24 June 2011 - 05:06 AM