Stable Cell Line generation
Posted 22 June 2011 - 09:18 AM
I also have pEGFP plasmid that I know very little about. It was Kanamycin resistant when I prepped the DNA. What should I use to try to select for it after transfection?
One more dilemma, do you usually do a kill curve for puromycin or is there a standard amount you should use? I have plasmid with the pKH vector that uses it.
Do you have a site that is a good reference place for someone with a degree in chemistry trying to work very diligently in cell biology???
Posted 22 June 2011 - 07:55 PM
Do you have a method to check that your transfection is working? If so - use this to test appropriate DNA:transfection reagent conditions for the HeLa.
The pEGFP plasmids I know don't have any eukaryotic selection markers on them.
Posted 22 June 2011 - 08:14 PM
Edited by leelee, 22 June 2011 - 08:16 PM.
Posted 23 June 2011 - 07:30 AM