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Nonisotopic northern blot probes


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#1 Natalie ;)

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Posted 22 June 2011 - 07:05 AM

Hi,
I am in process of preparing a northern blot and my current lab does not have a radioactive permit. I have never prepared a norther probe using a non-radioactive label. I was wondering if anyone used a DIG system for labeling the probes? How is the sensitivity with these systems? Or should I stick with radioactive isotopes?
I can't use qPCR since I'm looking for alternative variants of my transcript.

#2 phage434

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Posted 22 June 2011 - 07:19 AM

DIG works well, primarily limited by the sensitivity of your CCD imager (or film). I think there is very little reason for radioactivity in nucleic acid work (not true with metabolic compound labeling, e.g.).




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