Effect of long-term fixation on samples
Posted 21 June 2011 - 01:07 AM
I was wondering what the effect would be on my samples? What kind of staining methods can I still perform? I would like to do H&E staining and maybe some immunostaining. I doubt immunostaining will still work, but I would really appreciate some insight into this.
Thanks in advance!
Posted 21 June 2011 - 12:44 PM
but most likely no effect on your HE staining,
Immunostaining is depends on what epitope you will stain. Some IFC wont work or become very weak, if you fix too long.
for that reason, I normally fix only few hours.
Posted 21 July 2011 - 03:53 AM
i've left samples after fixation up to 5 weeks and they've been fine for embedding and sectioning. i still have some samples in the fridge that i didn't process and i believe they would still be ok to process in case it was necessary. according to some protocols, after the vaccuum infiltration you can leave your samples for months in the fixative, or in 100% ethanol.