hi. i will be digesting my gst fusion protein with thrombin. and it will yield a very low molecular weight protein of about 7kda. i would like to ask if there is a special technique of visualizing this protein in acrylamide gel? is 15% enough? or what other conditions do i need to consider in detecting this very low molecular weight protein? thank you....
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#2
silkworm
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Posted 20 June 2011 - 02:21 PM
Hello,
15% gel will do it. MES buffer will give you better resolution for LMW peptides.
15% gel will do it. MES buffer will give you better resolution for LMW peptides.
#3
Bluefunk
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Posted 25 June 2011 - 12:39 PM
15% might not give you enough separation. Check this out:
http://www.nature.co...prot.2006.4.pdf
These gels can clearly separate bands with a mass of 1 - 20 kDa
http://www.nature.co...prot.2006.4.pdf
These gels can clearly separate bands with a mass of 1 - 20 kDa
