I accidentally opened a sterile container of DMSO out of the tissue culture hood. It was sterile, so it was not supposed to be opened outside. I did not open it all the way and immediately closed the bottle back. What are the chances that it is contaminated?
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Opening a sterile reagent out of the tissue culture hood
Started by distalless, Jun 19 2011 06:05 PM
4 replies to this topic
#2
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Hmmm, I think it's working
Posted 20 June 2011 - 05:09 PM
The chances are actually fairly low... however do you want to take that risk???
#3
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Enthusiast
Posted 21 June 2011 - 12:54 AM
If you really want to use it: fill a tube with LB and a tube with YP, add your dmso and incubate at the appropiate temperatures, check after a few days for growth
#4
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Enthusiast
Posted 19 September 2011 - 02:24 PM
or, just sterile filter it....
#5
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Hmmm, I think it's working
Posted 19 September 2011 - 05:11 PM
Note - many filter types will dissolve in DMSO, choose a membrane type that doesn't!
