Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Opening a sterile reagent out of the tissue culture hood


  • Please log in to reply
4 replies to this topic

#1 distalless

distalless

    member

  • Active Members
  • Pip
  • 12 posts
1
Neutral

Posted 19 June 2011 - 06:05 PM

I accidentally opened a sterile container of DMSO out of the tissue culture hood. It was sterile, so it was not supposed to be opened outside. I did not open it all the way and immediately closed the bottle back. What are the chances that it is contaminated?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,784 posts
406
Excellent

Posted 20 June 2011 - 05:09 PM

The chances are actually fairly low... however do you want to take that risk???

#3 fysio lab

fysio lab

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
0
Neutral

Posted 21 June 2011 - 12:54 AM

If you really want to use it: fill a tube with LB and a tube with YP, add your dmso and incubate at the appropiate temperatures, check after a few days for growth

#4 briguy7

briguy7

    member

  • Active Members
  • Pip
  • 24 posts
1
Neutral

Posted 19 September 2011 - 02:24 PM

or, just sterile filter it....

#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,784 posts
406
Excellent

Posted 19 September 2011 - 05:11 PM

Note - many filter types will dissolve in DMSO, choose a membrane type that doesn't!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.