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brain tissue preparation for immunoblot

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#1 Inmost sun

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Posted 19 June 2011 - 02:48 AM

how do you dissect brain tissue for immunoblot analyzis, especially if you like to analyze phsophoproteins? I do not mean LASER dissection but with scalpel and binocular... any suggestion is welcome!

#2 bob1


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Posted 19 June 2011 - 05:03 PM

I'd freeze it and section with a cryotome then dissect out the bits wanted using a stereomicroscope. Note: I have never done this, but if I had to do it, this is probably how I would go about it.

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