i am doing FACS analysis for quite some time but not getting good result as expected by my boss.
i am checking the expression level of my receptor protein after bacterial infection compared to no infection .
i am getting very slight inc in expression .
i am attaching a file for that .
but my boss is expecting a more % of shift.
is my data valid or not .
attaching some file facs data 2.BMP 215.3KB 256 downloads