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how many samples should i prepare?


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#1 yimao

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Posted 17 June 2011 - 09:20 PM

Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!

#2 Inmost sun

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Posted 19 June 2011 - 02:55 AM

your request misses the aim of your 2D study so it is difficult to answer


generally you should run at least n=3 gels of 3 different cell cultures



Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!



#3 yimao

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Posted 19 June 2011 - 09:28 AM

oh, sorry about my bad expression.

i am trying to compare the protein expression differences made by culture cell in difference drug treat conditions.

do you mean i need to run 3*3=9 gels for one cell culture condition or 1*3=3 gels?

thx~

your request misses the aim of your 2D study so it is difficult to answer


generally you should run at least n=3 gels of 3 different cell cultures




Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.

May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?

Thanks!






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