Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.
May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?
Thanks!
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2 replies to this topic
#2
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Posted 19 June 2011 - 02:55 AM
your request misses the aim of your 2D study so it is difficult to answer
generally you should run at least n=3 gels of 3 different cell cultures
generally you should run at least n=3 gels of 3 different cell cultures
yimao, on 17 June 2011 - 09:20 PM, said:
Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.
May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?
Thanks!
May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?
Thanks!
#3
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Posted 19 June 2011 - 09:28 AM
oh, sorry about my bad expression.
i am trying to compare the protein expression differences made by culture cell in difference drug treat conditions.
do you mean i need to run 3*3=9 gels for one cell culture condition or 1*3=3 gels?
thx~
i am trying to compare the protein expression differences made by culture cell in difference drug treat conditions.
do you mean i need to run 3*3=9 gels for one cell culture condition or 1*3=3 gels?
thx~
Inmost sun, on 19 June 2011 - 02:55 AM, said:
your request misses the aim of your 2D study so it is difficult to answer
generally you should run at least n=3 gels of 3 different cell cultures
generally you should run at least n=3 gels of 3 different cell cultures
yimao, on 17 June 2011 - 09:20 PM, said:
Hi, i am plan to do a 2-DE experiment on cells cultured in different conditions.
May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?
Thanks!
May I ask how many sample/s do i need to prepare for one condition (of cell culture)? Because some papers said that we will need to run 3 gels for "each sample", is that means i need to collect three samples from cell culture or only need to collect one cell sample, but run 3 gels by proteins extracted from this sample?
Thanks!
