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What is the expected pattern of restriction digestion of human genomic DNA?


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#1 TracyDuke

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Posted 17 June 2011 - 12:07 PM

I am trying to do long distance inverse PCR (LDI-PCR), which requires restriction digestion of genomic DNA. I tried digesting my genomic DNA with BglII, XbaI and BamH1. I have the picture of the agarose gel attached to show you how it looks like after 2 hours digestion. gDNA1-4 are from the same cell type just different way of DNA isolation. Could anybody tell me if my digestion worked? Or what a good digestion pattern should look like? Thanks so much!

#2 phage434

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Posted 17 June 2011 - 01:16 PM

The picture didn't come through, but you should expect a smear, centered around about 4 Kb, the expected length of random DNA sequences cut with a six base cutter.

#3 TracyDuke

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Posted 21 June 2011 - 12:02 PM

Thank you so much, Veteran!




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