collecting cells HT1080
Posted 17 June 2011 - 07:06 AM
Posted 17 June 2011 - 07:25 AM
I always had an alternate hypothesis....
Posted 19 June 2011 - 04:47 PM
Posted 20 June 2011 - 07:04 AM
Or lysis directly in wells, so firt washing with PBS, then incubation 30 min on ice with lysis buffer, and then collecting and centrifuging? I was afraid that to collect cells with lysis buffer because it is with detergent, so I will have foam etc.
Posted 20 June 2011 - 05:07 PM
Don't worry about a little bit of foam, you should be able to pick up most of the cells without too much pipetting up and down.