I am facing a problem with quantification of real-time PCR data using rotor gene-Q machine. I am using this technology for the first time. Please suggest me what are the preliminary steps required to initiate the work.
I've isolated RNA from plant at three different stage of heat treatment and want to compare the results of all. All the kit were bought from Qiagen. In my experiment tubulin was used as standard but when I go for auto detect threshold level the program say that at least two standards are required to find auto detect threshold level. I've complemented my analysis with all the treatment using 24 targeted gene with one standard. Could you please suggest me how should I process my data for final analysis to publish in scientific journal.
How to analyze data?
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