I am trying to do cDNA library but it did not work with me at all I always end up with religated vectors, I begin with as much as 2-5 ug of total RNA, is it too low and how much RNA is enough to do it successfully?
I do it with adaptors, can I amplify my cDNA with adaptors by PCR?
please do not suggest any kits because it will take several months to get it?
do any one have any simple and guaranteed protocol to do it?
many thanks in advance
failure after another doing cDNA library
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