3 replies to this topic

[Krudex]

Hi,

As my topic title has said, can UV-induced Apoptosis on isolated mitochondria be done? The reason for this question is that I have to induce apoptosis and isolate mitochondria for AFM imaging.
So if my original plan cannot be done, would the reverse works say that I induce apoptosis, lyse the cell, fix (4% paraformadehyde + 0.1% glutaraldehyde) the mitochondria at certain time interval, then isolate the mitochondria?

Thank you for reading and your kind replies.

[bob1]

I would say that you would have a much better chance with the second option. I am not sure that you could induce apoptosis of the mitochondria isolated from the cell; surely you would need other cellular pathways to trigger it.

[Krudex]

Thanks for the reply. What do you think if I try to isolate the mitochondria and add in recombinant Bax protein to achieve the mitochondrial outer membrane permeabilization effect, instead of trying to trigger apoptosis?

[bob1]

That sounds possible, but will take quite a bit of optimisation to get working, I would have thought.