UV-induced Apoptosis on isolated mitochondria, can it be done?
Posted 15 June 2011 - 11:11 PM
As my topic title has said, can UV-induced Apoptosis on isolated mitochondria be done? The reason for this question is that I have to induce apoptosis and isolate mitochondria for AFM imaging.
So if my original plan cannot be done, would the reverse works say that I induce apoptosis, lyse the cell, fix (4% paraformadehyde + 0.1% glutaraldehyde) the mitochondria at certain time interval, then isolate the mitochondria?
Thank you for reading and your kind replies.
Posted 16 June 2011 - 03:52 PM
Posted 16 June 2011 - 11:40 PM
Posted 19 June 2011 - 04:52 PM
Posted 28 August 2012 - 11:25 PM
For example, I tried to deposit mitochondria on compact disc having holes of approx. 200nm height but unfortunately mitochondria did not attach to the disc.