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Question regarding pellet sticking on tubes


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2 replies to this topic

#1 Redtuna

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Posted 15 June 2011 - 05:26 PM

Trying to centrifuge down some bone marrow cells (previously treated with radiation) (with 60 sec, highest speed on the small centrifuge) cells are in 1 ml PBS (and with a concentration of 10^6 cells/ml) in 1.5 ml microcentrifuge tubes... after spinning, nothing occurred.. then increased spinning times, did not have much improvement. any suggestions? Thank you guys!

#2 pcrman

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Posted 19 June 2011 - 08:42 PM

You said the pellet stuck to the tubes? Are you using a fixed angele rotor or swing bucket rotor? If you use the latter, you will get the pellet at the bottom of the tube.

#3 Rnotk

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Posted 20 June 2011 - 02:43 PM

nothing happen?? what do you mean?
I do work with BM, but it should become pellet, maybe you just cannot see the pellet




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