Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Isothermal DNA amplification


  • Please log in to reply
3 replies to this topic

#1 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 707 posts
28
Excellent

Posted 15 June 2011 - 09:05 AM

There are quite a number of Isothermal DNA amplification techniques.
This includes LAMP, HDA, RPA and etc.

Did anyone of you use it here? Do it have any values in your research? Synthetic Biology? Molecular Diagostic?

How do you think?

I had read some publications saying that such technology is better/not good than conventional PCR. But how come people were so phobia about PCR anyway?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,710 posts
123
Excellent

Posted 15 June 2011 - 10:37 AM

how come people were so phobia about PCR anyway?


cost of setting up (thermal cycler).
talent does what it can
genius does what it must
i do what i get paid to do

#3 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,508 posts
94
Excellent

Posted 15 June 2011 - 12:33 PM

here is a bioforum thread were it was a topic already, though not that long....
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#4 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 707 posts
28
Excellent

Posted 15 June 2011 - 07:16 PM

here is a bioforum thread were it was a topic already, though not that long....

Hi Hobglobin, can PM me the paper?

Btw, did anyone had any experience in doing such work before?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.