I have bottle of IL2. it is said to dilute in 100mM acetic acid to a final concentration of 100ug/ml atleast. Now I need how many units would that be. And how to dilute that in 100mM acetic acid.
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#2
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Posted 14 June 2011 - 02:24 PM
let me make it clear. I have IL 2 in a bottle of 5ug. I need to dilute it in 100mM acetic acid to a final concentration of not less than 100 ug/ml. The activity of it is 5*10^6 U/mg.
Now I need 2000 U of il-2 for my experiment. So, how much volume of acetic acid I should dilute it in to get a stock of say 500U per ul?
Now I need 2000 U of il-2 for my experiment. So, how much volume of acetic acid I should dilute it in to get a stock of say 500U per ul?
#3
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Hmmm, I think it's working
Posted 14 June 2011 - 04:41 PM
So you have 5,000,000 U/mg or 5,000 U/ug and 5 ug... you have 2,5000 U total. To get 500 U/ul V=N/C = 2,5000/500 =...
