Sybr green RT-qPCR primer
Posted 14 June 2011 - 07:28 AM
Besides that, one step RT-qPCR or two-step RT-qPCR is of better choice if i want to study lots of interlukine genes(around 15 genes) from a tissue sample?
Finally, can someone share with me on the flow of running the RT-qPCR? (A simple one will help me lots as i have read lots journal and i actually end up with confious)
Posted 16 June 2011 - 07:40 AM
What exactly are you asking about? What to do first when your primers arrive? How to optimise the reaction? How to pipette it? How to prepare lab for doing qPCR?
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