2 replies to this topic

[UncleRudder]

I would like to do a study looking at cellular uptake rates using fluorescence.  I understand that the standard plate to use for fluorescence studies is a 96 well plate with clear bottom and black sides.  However, these plates are quiet expensive and we have a boat load of clear bottom, white side 96 well plates that have we have typically used for luminescence.  Since the walls are opaque white instead of opaque black I would venture a guess that they would work better for blocking out interfence between wells than a clear 96 well plate, so can they be used in place of the similar black plates?  I have done a few trial runs and the plates show no auto-fluorescence and seem to work well.  Thoughts?  

Thanks in advance!

[newborn]

My experience on this problem is you can use black and opaque (normal plate) for fluorescene. The while plate enhances the intensity that make your baseline too high.

[asli]

newborn, on 21 June 2011 - 12:06 AM, said:

My experience on this problem is you can use black and opaque (normal plate) for fluorescene. The while plate enhances the intensity that make your baseline too high.

I have been doing a very similar cellular uptake study with fluorophores in regular clear TC treated 96-well plates, but since my signal levels are low, the high background signal from the Negative controls or even empty wells is a big problem.
I am planning to buy black 96-well plates, but I don't know if they should be glass at the same time..I won't do imaging studies in a microscope, I'll just get the endpoint reading in a fluorescence microplate reader (SpectraMax) in bottom read mode.

Any suggestions or comments?
What brand is best or good and relatively inexpensive?

Thanks in advance for any help.

Edited by asli, 04 August 2011 - 02:01 AM.