Using White 96 Well Plates for Fluorescence Study
Posted 14 June 2011 - 06:25 AM
Thanks in advance!
Posted 21 June 2011 - 12:06 AM
Posted 04 August 2011 - 02:00 AM
My experience on this problem is you can use black and opaque (normal plate) for fluorescene. The while plate enhances the intensity that make your baseline too high.
I have been doing a very similar cellular uptake study with fluorophores in regular clear TC treated 96-well plates, but since my signal levels are low, the high background signal from the Negative controls or even empty wells is a big problem.
I am planning to buy black 96-well plates, but I don't know if they should be glass at the same time..I won't do imaging studies in a microscope, I'll just get the endpoint reading in a fluorescence microplate reader (SpectraMax) in bottom read mode.
Any suggestions or comments?
What brand is best or good and relatively inexpensive?
Thanks in advance for any help.
Edited by asli, 04 August 2011 - 02:01 AM.