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how to do the antibody supershift through PAGE gel


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#1 shockie

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Posted 19 September 2002 - 11:53 PM

r there anybody doing supershift now?I'm in the dilemma now.IThe supersift band cann't be viusialized on the PAGE gel after phosphor sreezning.Would you pls tell me the method soon? My steps is followed: mix binding buffer(promega),ddH2O, nuclear extrat for ten miniutes at room temperature then add appropriate olig probe , mix for 30 minutes under RT,then add target protein apecific antibody for 20 minutes.
what's wrong with the above method?
tks

#2 snowman

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Posted 24 September 2002 - 05:00 AM

do you use a non-denaturating gel?

#3 shockie

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Posted 26 September 2002 - 02:22 AM

yes




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