Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Citrate buffer without citric acid?


  • Please log in to reply
3 replies to this topic

#1 dustbunny000

dustbunny000

    member

  • Active Members
  • Pip
  • 8 posts
1
Neutral

Posted 13 June 2011 - 12:46 PM

I want to make citrate buffer (pH 3.8). As I understand you need both trisodium citrate and citric acid to prepare this buffer. However we do not have citric acid on hand and I think in the past this buffer was prepared without citric acid. Do you really need citric acid? Wouldn't it be just as affective to pH with HCl?

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,418 posts
238
Excellent

Posted 13 June 2011 - 03:47 PM

The difficulty of using HCl is that you (in addition to your buffer) have also made salt from the sodium chloride formed. This may or may not be an issue in your application, but you should be aware of its presence.

#3 dustbunny000

dustbunny000

    member

  • Active Members
  • Pip
  • 8 posts
1
Neutral

Posted 14 June 2011 - 05:52 AM

Yeah, I thought about that! But the ionic strength in my experiments is already going to be quite high so I don't think a little extra should be a problem. Perhaps trying it both ways would give me more peace of mind...

#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,744 posts
127
Excellent

Posted 15 June 2011 - 09:48 AM

as long as the addition of a little additional salt won't have an adverse effect on your system then you can take trisodium citrate and adjust the pH with acid. keep in mind that the citrate concentration will change with the addition of the acid so adjust the pH before bringing to final volume.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.