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EB too confluent?


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#1 mingjietong

mingjietong

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Posted 13 June 2011 - 11:03 AM

Hi Guys,

I am following 2004 Nature Neuroscience paper to make neurons ( Volume 7 Number 9 Page 1003 "Differentiation of mouse embryonic stem cells into a defined neuronal lineage").

The attached picture is EB after 7 days, 4 days in ES medium and 3 days in ES + 5 uM RA. Does it look too confluent? They look very dark under scope and aggregate into big clumps.

Thanks

Attached Thumbnails

  • EB_Nature_7D_c6.JPG





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