Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Human B-lymphocyte expansion


  • Please log in to reply
2 replies to this topic

#1 neuropath

neuropath

    member

  • Active Members
  • Pip
  • 25 posts
1
Neutral

Posted 13 June 2011 - 01:45 AM

Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath

#2 monkee

monkee

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 June 2011 - 05:15 AM

Hi neuropath, I have little experience on lymphocyte culture but came upon this article after a bit of googling.. I hope this is close to what you need :)

Wiesner M, Zentz C, Mayr C, Wimmer R, Hammerschmidt W, et al. (2008) Conditional Immortalization of Human B Cells by CD40 Ligation. PLoS ONE 3(1): e1464. doi:10.1371/journal.pone.0001464

monkee

Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath



#3 neuropath

neuropath

    member

  • Active Members
  • Pip
  • 25 posts
1
Neutral

Posted 13 June 2011 - 01:40 PM

Hi monkee,

This is great! Thanks a lot for your help! :D

neuropath

Hi neuropath, I have little experience on lymphocyte culture but came upon this article after a bit of googling.. I hope this is close to what you need :)

Wiesner M, Zentz C, Mayr C, Wimmer R, Hammerschmidt W, et al. (2008) Conditional Immortalization of Human B Cells by CD40 Ligation. PLoS ONE 3(1): e1464. doi:10.1371/journal.pone.0001464

monkee


Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.