2 replies to this topic

[neuropath]

Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath

[monkee]

Hi neuropath, I have little experience on lymphocyte culture but came upon this article after a bit of googling.. I hope this is close to what you need

Wiesner M, Zentz C, Mayr C, Wimmer R, Hammerschmidt W, et al. (2008) Conditional Immortalization of Human B Cells by CD40 Ligation. PLoS ONE 3(1): e1464. doi:10.1371/journal.pone.0001464

monkee

neuropath, on 13 June 2011 - 01:45 AM, said:

Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath

[neuropath]

Hi monkee,

This is great! Thanks a lot for your help!  

neuropath

monkee, on 13 June 2011 - 05:15 AM, said:

Hi neuropath, I have little experience on lymphocyte culture but came upon this article after a bit of googling.. I hope this is close to what you need

Wiesner M, Zentz C, Mayr C, Wimmer R, Hammerschmidt W, et al. (2008) Conditional Immortalization of Human B Cells by CD40 Ligation. PLoS ONE 3(1): e1464. doi:10.1371/journal.pone.0001464

monkee

neuropath, on 13 June 2011 - 01:45 AM, said:

Hi,

I am trying to expand B lymphocytes in mixed lymphocytes isolated from human peripheral blood. I have found protocols for expanding T-lymphocytes but not for B-lymphocytes. I want to amplify the B lymphocyte numbers in general and necessarily for cytogenetics study. Would appreciate it greatly if anyone can share a working protocol.

Thanks!

Neuropath