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Separating protein from sucrose by precipitation?


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#1 and then there were none

and then there were none

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Posted 06 September 2002 - 09:09 AM

hi everyone,

I've been purifiying rafts by sucrose gradients and I need to concentrate the fractions. I've tried acetone and acetone precipitation. However, it seems that the sucrose gets precipitated to form a lower heavy layer along with the protein in the bottom of the tube used to precipitate protein. Is there any way of separating protein from sucrose? I thought people use acetone to precipitate protein and also to get rid of polysaccharide from protein samples for 2-D gel sample preparations. How can I separate protein from sucrose?

thank you!

David

#2 lifengzhang

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Posted 05 March 2003 - 03:47 PM

try use phenol to extract your protein and precpitate with methonal




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