I know that one should use an over night culture for plasmid DNA preparations. The culture is supposed to be 12-16 hours. I have never gotten any explanation of why not 10 or 18 hours?
Maybe the reason is very obvious but I don't see it. Could anyone enlighten me?
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#2
bob1
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Posted 10 June 2011 - 08:29 PM
Too short an incubation to get a big amount of DNA, and by 18 hours the cells will usually have stopped propagating in a logarithmic fashion - so you get less plasmid yield respectively.
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AnetteM
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Posted 11 June 2011 - 12:33 AM
Thanks. I figured it was something like that but I thought that maybe there was more to it such as an effect on DNA if the cells were "too old".
