Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Rehydration procedures for preserved fish

  • Please log in to reply
No replies to this topic

#1 wolfyFTW



  • Members
  • Pip
  • 1 posts

Posted 10 June 2011 - 10:02 AM

Hello everyone, just registered to pose a techniques question.

In my lab I am dissecting various species of fish and weighing their individual organs. The fish I am working with have been initially fixed in formalin and then transferred to 70% ethanol. However, the ethanol quickly evaporates during the dissection and weighing stages, preventing me from obtaining accurate measurements. I was thinking of gradually transferring the fish from 70% ethanol --> 50% ethanol --> 30% ethanol --> 15% ethanol --> 0% ethanol (all in 0.1M phosphate buffer, too), but I'm not sure how long I should keep the fish in each solution before transferring it to the next. I want to give them enough time to rehydrate. Would 24 hours suffice? These fish all weigh between 10 and 20 grams, so they're not very large


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.