Hello everyone, just registered to pose a techniques question.
In my lab I am dissecting various species of fish and weighing their individual organs. The fish I am working with have been initially fixed in formalin and then transferred to 70% ethanol. However, the ethanol quickly evaporates during the dissection and weighing stages, preventing me from obtaining accurate measurements. I was thinking of gradually transferring the fish from 70% ethanol --> 50% ethanol --> 30% ethanol --> 15% ethanol --> 0% ethanol (all in 0.1M phosphate buffer, too), but I'm not sure how long I should keep the fish in each solution before transferring it to the next. I want to give them enough time to rehydrate. Would 24 hours suffice? These fish all weigh between 10 and 20 grams, so they're not very large
Rehydration procedures for preserved fish
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