Hello everyone, just registered to pose a techniques question.
In my lab I am dissecting various species of fish and weighing their individual organs. The fish I am working with have been initially fixed in formalin and then transferred to 70% ethanol. However, the ethanol quickly evaporates during the dissection and weighing stages, preventing me from obtaining accurate measurements. I was thinking of gradually transferring the fish from 70% ethanol --> 50% ethanol --> 30% ethanol --> 15% ethanol --> 0% ethanol (all in 0.1M phosphate buffer, too), but I'm not sure how long I should keep the fish in each solution before transferring it to the next. I want to give them enough time to rehydrate. Would 24 hours suffice? These fish all weigh between 10 and 20 grams, so they're not very large
Thanks!
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