Tissues used to genotyping animals
Posted 09 June 2011 - 11:16 PM
What tissues does everyone here take for genotyping? For mice I've read the common one is either tail or toe snip. I'm about to do some behavioral work and am hesitant to essentially mutilate my mice.
Moreover, has anyone had success with using stool samples for genotyping purposes? There is an interesting paper on it, but I am unsure of how successful it would be for me: http://www.ncbi.nlm....pubmed/10580111
Posted 10 June 2011 - 01:01 AM
Posted 11 June 2011 - 04:47 PM
Basically you use a scalpel (and a glass bead sterilizer) and gently take one pup out of the nest at a time, cut off 1mm of tail tip, and then cut that piece in half again, thus getting two samples to test. The hot scalpel cauterizes the tail so it hopefully won't bleed...then we toe-tattoo each pup for identification. We have found that sampling from the rather young pups (eyes not quite even open yet) minimizes stress.
Posted 10 April 2012 - 07:23 PM