Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Tissues used to genotyping animals


  • Please log in to reply
3 replies to this topic

#1 Xanthier

Xanthier

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 09 June 2011 - 11:16 PM

Hi all!

What tissues does everyone here take for genotyping? For mice I've read the common one is either tail or toe snip. I'm about to do some behavioral work and am hesitant to essentially mutilate my mice.

Moreover, has anyone had success with using stool samples for genotyping purposes? There is an interesting paper on it, but I am unsure of how successful it would be for me: http://www.ncbi.nlm....pubmed/10580111

#2 Rsm

Rsm

    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 384 posts
9
Neutral

Posted 10 June 2011 - 01:01 AM

You can also use urine samples (at least you know exactly from which mouse the sample is, particularly when you have more than one mouse per cage). You'll need to "squeeze" the mouse a bit, don't know how much that affects your experiment...
I got soul, but I'm not a soldier

#3 Fungus_Dreams

Fungus_Dreams

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 36 posts
0
Neutral

Posted 11 June 2011 - 04:47 PM

We take tail snips from 10-12 day old mouse pups.

Basically you use a scalpel (and a glass bead sterilizer) and gently take one pup out of the nest at a time, cut off 1mm of tail tip, and then cut that piece in half again, thus getting two samples to test. The hot scalpel cauterizes the tail so it hopefully won't bleed...then we toe-tattoo each pup for identification. We have found that sampling from the rather young pups (eyes not quite even open yet) minimizes stress.

#4 AquaPlasmid

AquaPlasmid

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 74 posts
7
Neutral

Posted 10 April 2012 - 07:23 PM

Stool works well for genotyping transgenic mice. Here is a new article in Lab Anim (NY). 2012 Mar 20;41(4):102-7, Non-invasive genotyping of transgenic animals using fecal DNA. You'll get a few clean droppings by moving a mouse out of its cage. The DNA is extracted from a fecal dropping with 150 ul of AquaStool solution, costing about $1/extraction. The animals certainly appreciate not being amputated :D




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.