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[div]

Hi
I work with running real time PCR s on urine samples, sometimes bloody urine samples, lookin for for mRNA markers for cancer diagnosis. the bloody samples dont yield good Cts. Could you suggest additives/pcr enhancers?  I tried .1- 1 mM EDTA, hoping it will help chelate the iron in the blood n help the pcr. but it didnt. Many forums talk abt bsa and betaine etc, but does anyone know of any additive more relavant to real time RT PCR?

[Arantxa_Penya]

Hi,

I'm working with qPCR too and I have the same question. Can I use BSA for enhancer my qPCR? I read some references about this, but either related with qPCR. Anybody can help me?

thanks for all