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Annexin-V staining

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#1 Miss S

Miss S


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Posted 09 June 2011 - 12:04 PM

I am new to flow cytometry analysis, sorry if my question seems stupid. i am working with thymi and going to administer hidrocortisone to mice. i need to detect apoptosis by AnnexinV-FITC staining. But during thymocytes isolation I will damage cells (control cells also go through the same procedure), resulting more than expected number of PI +ve and annexin +ve cell. What do you think? I need advice before I do my research. Thank you in advance.
Miss S

#2 Denis Baev

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Posted 11 July 2011 - 11:12 AM

In thymus you ALWAYS have a huge % of apoptotic cells. It is normal.

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