could i use sterile deionized water instead of DEPEC water in RNA extraction
Posted 09 June 2011 - 11:13 AM
i am doing RNA extraction for doing RT-PCR but i dont have DEPEC in my lab.
so could i use autoclaved deionized water instead of DEPEC treated water
Posted 09 June 2011 - 07:15 PM
Long answer: it may be OK, but if you have any RNAse contamination in your deionised water supply, your RNA will be digested. Autoclaving will not prevent this - RNAses will renature.
You can buy RNAse free water if required.
Posted 10 June 2011 - 12:24 AM
Posted 10 June 2011 - 01:26 AM
But i have another question
what about the micrfuge tubes and pipette tips (plasticwares)should i wash them by DEPEC water before autoclaving???
Posted 10 June 2011 - 10:41 AM
genius does what it must
i do what i get paid to do
Posted 11 June 2011 - 03:44 PM
which water do u mean??????????????
(after soaking, you should also autoclave the water to eliminate the depc).
Posted 11 June 2011 - 04:08 PM