Measuring methylation on X chromosome of female subjects
Posted 09 June 2011 - 05:17 AM
I want to look at the methylation status of Foxp3, which is located on the X chromosome. All papers I have read say that they only look at male patients/mice, to avoid possible artifacts due to X linked inactivation in females. My problem is that the disease I am studying has a large female preponderance (>95% of my samples are female) and so I really need to measure methylation in female cells. Does anyone know of any way that I can overcome this problem? Or even how I can can calculate some sort of probability of how much the inactivation may impact on my results so I can at least defend myself when I come to publish? I am thinking that if at least look at quite a few patients, and compare to the same number of female controls, that I can make some sort of conclusions...?
Thanks for any advice you can give,
p.s. The method I am using is the SABiosciences (Qiagen) EpiTect Methyl qPCR Primer Assay for Human FOXP3
Posted 19 June 2011 - 08:37 PM
Posted 31 July 2011 - 05:47 PM
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 30 September 2011 - 12:59 PM