Brain dissection for immunoblot
Posted 28 November 2011 - 07:21 PM
yes it is a good question and do you have answer now?
how do you dissect brain tissue for immunoblot analyzis, especially if you like to analyze phsophoproteins? I do not mean LASER dissection but with scalpel and binocular... any suggestion is welcome!
Posted 10 January 2012 - 08:35 PM
one in each hand. Pluck out your sample (Paxinos brain atlas helps to learn morphology) and put it in a tube that is already in dry ice. I touch the sample to dry ice as soon as its out as well, to freeze it. This works fairly well for phopho proteins on western blot.